Behaviour after cardiosurgery

Aim of this study was to estimate behavioral changes after cardiac surgery (CS). A questionnaire was sent by mail to 74 women and 122 men during May 2004 and the answers were collected by phone. For statistical analysis, the chi2-test was used. A common unhealthy habits before CS were inappropriate diet (80% of all responders), addictions to alcohol (62%) and nicotine (54%). Significantly fewer patients after CS continue with fatty diet (chi2 = 39.069; p < 0.001), smoking (chi2 = 90.286; p < 0.001) and alcohol drinking habits (chi2 = 60.667; p < 0.001). A significant worsening of sexual life (chi2 = 91.533; p < 0.001) and significantly less negative influence of weather changes (chi2 = 20.821; p < 0.001) was found after CS. Men drink more (chi2 = 16.299; p < 0.001) and smoke more (chi2 = 19.635; p < 0.001), have better sexual life (chi2 = 17.317; p < 0.001), fewer sleeping disorders (chi2 = 17.334; p < 0.001) and better oral health (chi2 = 38.632; p < 0.001) than women. These results can be useful for setting up preventive measures for CVD.     

The influence of mouse sera, regenerating liver extracts and bacterial products on the abilities of different cells in vitro.

In the complexity of host tumor relations, the regeneration of the tissue in which the tumor is growing, or in some other tissue in the organism, could influence the maturation of tumor cells, i.e. tumor reversion. Clinical observations and experiments on plants, lower animals, or animal embryos, performed by several authors, and our results on the influence of regenerating mouse liver on the abilities of tumor transplanted there or elsewhere in the organism led us to study the in vitro growth of different cells or bacteria exposed to the extracts of normal or regenerating liver and/or sera from these animals. Further, sterile used bacterial media were added to bacterial or cell cultures, respectively. Depending on the model, liver extracts-particularly extracts and sera from mice with regenerating liver-were shown to inhibit radioactive thymidine incorporation in the cells. In these experiments, the number of bacteria or cells per culture was lower than in otherwise treated corresponding cultures. Further, used sterile media of bacterial cultures stimulated the growth of bacteria but inhibited thymidine incorporation into fibrosarcoma cells in vitro. Whether this means that one or several common regulators exist in nature appears as an intriguing, but still completely open question. The idea of controlling tumor growth by using such regulatory growth factors seems very provocative.     

Improved method of canine decerebration

We describe animproved decerebration method for dogs that is suitable for studies ofbrain stem neurons in the absence of anesthesia. Previously reportedtechniques of canine decerebration often lead to respiratory andhemodynamic instability and lack of typical decerebrate rigidity. Wehave developed a precise, visually controlled, midcollicular brain stemtransection technique that overcomes these problems. Our method resultsin only moderate blood loss while preserving carotid and basilar arterycirculations. Consistent levels of brain stem transection routinelylead to stable postdecerebration hemodynamic parameters, allowingprolonged brain stem neuronal recordings. The same model should also be useful for a variety of studies involving other physiological systemsin dogs in the absence of anesthesia and for studies of anestheticeffects.

     

High-throughput immunoaffinity enrichment and N-glycan analysis of human plasma haptoglobin

Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N-glycosylation sites carrying mainly complex type N-glycans. Its glycosylation is altered in different types of diseases but still has not been extensively studied mainly due to analytical challenges, especially the lack of a fast, efficient, and robust high-throughput Hp isolation procedure. Here, we describe the development of a high-throughput method for Hp enrichment from human plasma, based on monolithic chromatographic support in immunoaffinity mode and downstream Hp N-glycome analysis by hydrophilic interaction ultrahigh-performance liquid chromatography with fluorescent detection (HILIC–UHPLC–FLR). Chromatographic monolithic supports in a 96-well format enable fast, efficient, and robust Hp enrichment directly from diluted plasma samples. The N-glycome analysis demonstrated that a degree of Hp deglycosylation differs depending on the conditions used for N-glycan release and on the specific glycosylation site, with Asn 241 being the most resistant to deglycosylation under tested conditions. HILIC–UHPLC–FLR analysis enables robust quantification of 28 individual chromatographic peaks, in which N-glycan compositions were determined by UHPLC coupled to electrospray ionization quadrupole time of flight mass spectrometry. The developed analytical approach enables fast evaluation of total Hp N-glycosylation and is applicable in large-scale studies.     

Yield performance of 14 novel inter- and intra-species Miscanthus hybrids across Europe

Miscanthus, a C4 perennial rhizomatous grass from Asia is a leading candidate for the supply of sustainable biomass needed to grow the bioeconomy. European Miscanthus breeding programmes have recently produced a new range of seeded hybrids with the objective of increasing scalability to large acreages limited by current clonal propagation. For the EU-GRACE project, new replicated field trials were established in seven locations across Europe in 2018 with eight intraspecific M. sinensis hybrids (sin × sin) and six M. sacchariflorus × M. sinensis (sac × sin) from Dutch and UK breeding programmes, respectively, with clonal Miscanthus × giganteus. The planting density of the sin × sin was double that of sac × sin (30,000 & 15,000 plants ha⁻¹), creating commercially relevant upscaling comparisons between systems. Over the first 3 years, the establishment depended on location and hybrid. The mature sin × sin hybrids formed tight tufts of shoots up to 2.5 m tall which flower and senesce earlier than the taller sac × sin hybrids. Following the third growing season, the highest yields were recorded in Northern Italy at a low altitude (average 13.7 (max 21) Mg DM ha⁻¹) and the lowest yielding was on the industrially damaged marginal land site in Northern France (average 7.0 (max 10) Mg DM ha⁻¹). Moisture contents at spring harvest were lowest in Croatia (21.7%) and highest in Wales, UK (41.6%). Overall, lower moisture contents at harvest, which are highly desirable for transport, storage and for most end-use applications, were found in sin × sin hybrids than sac × sin (30% and 40%, respectively). Yield depended on climate interactions with the hybrid and their associated planting systems. The sin × sin hybrids appeared better adapted to northern Europe and sac × sin hybrids to southern Europe. Longer-term yield observations over crop lifespans will be needed to explore the biological (yield persistence) and economic costs and benefits of the different hybrid systems.     

Loss and recovery of transplantability of L-P59 cells

Summary After cultivation in vitro for 3 years, L-P59 cells failed to produce tumors in C3Hf/Bu mice. Meanwhile, the cells retained their antigenic abilities, i.e. they induced resistance in the recipient to subsequent challenge with tumorigenic L-P59 cell line. After 2 weeks of cultivation in agar, those nontumorigenic cells became again able to transplant the tumor in C3Hf/Bu recipients. This work was supported in part by the United States Public Health Service Grants CA 05047 and CA 6294.